Coding

Part:BBa_K5226043

Designed by: Yujiao Yang   Group: iGEM24_SCUT-China-A   (2024-09-22)


stth

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1084
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 213
    Illegal NgoMIV site found at 471
    Illegal NgoMIV site found at 1174
    Illegal NgoMIV site found at 1440
    Illegal AgeI site found at 359
    Illegal AgeI site found at 700
  • 1000
    COMPATIBLE WITH RFC[1000]


Introduction

One of the goals of iGEM SCUT-China-A is to use synthetic biology tools to obtain Halomonas strains that can produce tyrian purple. We chose to introduce four enzymes that is either necessary or beneficial to the production of tyrian purple.

Usage and Biology

Stth is a kind of tryptophan 6-halogenase. The protein sequence is from Streptomyces toxytricini. This protein catalyzes the conversion of tryptophan (Trp) into 6-bromotryptophan(6-Br-Trp).


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